Question

what mechanisms/machineries may be inhibited if the CTD tail has mutations? What problems could be encountered in RNA processing in these cell lines if there are mutations? list four things that can go wrong with RNA processing if there is a problem with the CTD tail.

Answer 1

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Genetic Information encoded in DNA transcribed into mRNA by RNA polymerase II. Transcription involves the synthesis of pre-mRNA and the processing of pre-RNA into mRNA.

Pre-mRNA and mRNA exhibit differences at the structural level. For instance; mRNA contains 5’ CAP and poly(A) tail. Intron sequences spliced out from pre-mRNA to generate mRNA. mRNA contains only exon sequences. Above mentioned processes (addition of polyA tail, addition of 5’ CAP and removal of intron sequence) are regulated by CTD of RNA polymerase II.

Hence, a mutation in CTD would affect the formation of 5’cap, splicing, and 3′ cleavage and polyadenylation processes. Eventually, CTD mutation would affect the generation of mRNA