Which of the following is NOT based on antigen-antibody complexes?
A) Serology
B) Western blotting
C) Direct ELISA test
D) Indirect ELISA test
E) Phage Typing
Phage typing
ELISA is enzyme-linked immunosorbent assay. It is used for the detection of specific antibodies in a given sample. It is based upon the interaction between antigen and antibody. The result is the presence of a coloured product if there is interaction between antigen and antibody and if no colour is formed, then antibodies are absent in the given sample.
Serology is also based upon the interaction between antigen and antibody.
Western blotting is a technique of detection of specific proteins in a given sample using specific probe. This is also based upon antigen antibody interaction.
All these three methods are used routinely in immunology.
Phage typing is a method in microbiology which is used for the detection of single strain of bacteria on the principle that some bacteriophages infect only a specific strain of bacteria.
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