Question

How can you determine what wavelength was used after calculating the absorbance through the extinction coefficient

I used A=Ebc to get the absorbance, but how do i now calculate the wavelength used?

Answer 1

The value for E, which stands for molar attenuation coefficient, is an intrinsic property which is different for every wavelength. You cannot directly calculate wavelength from the terms in this equation, as it is assumed that E was measured at a known wavelength, and the resulting value was used in the equation. In biology, the wavelengths 280 nm and 260 nm are the ones used the most for proteins and nucleic acids respectively, so check with the original question for the type of molecule in question.