Question

2. Enzymase (protein with 120 amino acids) catalyzes a critical reaction in the cell at pH...

2. Enzymase (protein with 120 amino acids) catalyzes a critical reaction in the cell at pH 7.2. The protein contains a Glu residue at #45 (Glu^45) that forms an essential ionic interaction with Lys^72. Loss of this interaction result in no enzyme activity.

A. Draw the essential parts of the involved amino acids showing the key interaction at pH 7.2

B. If the Cellular pH inccreases to 9.5, what effect will this have on the activity of enzymase? Explain, including a drawing of the key interaction at this pH if helpful.

C. If the cellular pH decreases to 3.0 what effect will this have on the activity of enzymase? Explain, including a drawing of the key interaction at this pH if helpful.

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