Question

What are some limitations of RNA-interference based experimentation in molecular biology?

Why might RNA interference only take place post transcription? And would there be a way to test experimentally exactly when RNA interference occurs?

Answer 1

Solution

Limitations

1. it eliminates gene expression throughout the entire organism, and cannot be used for studying developmental or cell type–specific effects unless tissue-specific knock-outs are employed.
2. It is generating knock-out models is both labor and time intensive.

• RNA interference of posttranscriptional gene silencing, cosuppression, quelling, and RNAi re follows a similar core pathway which are workout at after tranacription.
• It is required to silence gene at before translation and after tranacription because RNA interference process ia transcription and RNA based.

Way of testing

• Microarray experiments revealed that chemically synthesized siRNAs trigger the activation of pathways for gene silencing.