Question

Which of the following correctly associates the RNAi mechanism with its property?

miRNAs typically show perfect complementarity with their target sequence

siRNAs typically cleave their target RNAs using the slicing activity of the Argonautes

miRNAs can recruit a RITS, but not a RISC complex

miRNAs are a more ancient form of gene regulation because of their role in cellular immunity

Answer 1

Ans) The correct answer is "B" i.e siRNAs typically cleave their target RNAs using the slicing activity of the Argonautes (Argonaute proteins are endonucleases and they cleave the target mRNA which is complementarily to the bound siRNA (guide strand) and this is how gene silencing will occur wheras the passenger strand will be degraded during RISC activation)

Option A is partially correct. Plants have miRNAs which can perfectly base pair with the target mRNA.But this does not happen in animals.

Option C is incorrect because miRNA can recruit RISC complex (dicer process pre-miRNA to miRNA duplex and then loaded into RISC).