Question

Since one of Lwoff's suggestions is that a virus should be produced solely from its nucleic acid, purified viral nucelic acids genomes released free in extra cellular space of susceptibles, permissive cells are infectious

A: True or False

If you added a cap to the 5'-end and poly-A track to the 3'-end of Sendai virus vRNA the resulting RNA will be translated in a rabbit reticulocyte cell-free translation system

A: True or False

Answer 1

Ans- False

Explanation- A virus can only replicate by insertion of its genomic material into host genome. For this, virus requires to be attached to host cells that is possible only by its protein coat and then entry of genome into host cell, therefore, free nucleic acid of virus will not be able to enter host and hence, not be infectious.

Ans- True

Explanation- RNA of virus with 5'end and poly A tail will be identified as eukaryotic mRNA and hence, be translated in rabbit.