Question

After the fusion of the viral and endosomal membranes, the negative-stranded genome segments of the influenza virus are released into the cytoplasm.  Describe what happens to genome segments 4, 5, and 7 during the viral replication cycle.

• Where they are localized
• How the gene products they encode are expressed, including all viral and cellular proteins that are involved
• How each of the gene products encoded by each segment are trafficked through the infected cell, including each of the cellular compartments involved in the trafficking
• Where each of the gene products end up in the progeny virions

Answer 1

THE GENOME 4,5 ENCODE FOR ONE PROTEIN PER SEGMENT. ( HA AND NA PROTEINS) AND SEGMENT 7 ENCODE FOR MI PROTEINS.

REPLICATION OF THE GENOME DOES NOT REQUIRE A PRIMER, INSTEAD, THE VIRAL RNA DEPENDENT RNA POLYMERASE (RdRp) INITIATES RNA POLYMERASE.

THE ENVELOP OF PROTEIN AA, NA, M2 ARE SYNTHESISED FROM MRNA OF VIRAL ORGININ.ON MEMBRANE RIBOSOME into ER, WHEN THEY WILL BE FOLDED AND TRAFFICKED TO GOLGI APPARATUS FOR POST TRANSLATIONAL CHANGES.

VIRUS BUDDING OCCURS AT THE CELL MEMBRANE, BY AN ACCUMULATION OF MI MATRIX PROTEIN INCYTOPLASMIC LAYER ,WHEN BUDDING IS COMPLETED ,HA SPIKES CONTINUE TO BIND THE VIRIONS TO SIALIC ACID ON CELL SURFACE UNTIL VIRAL PARTICLES ARE RELEASED WELL.