Question

1. How can you be certain that you have regenerated somatic embryo and not shoot? What structural features differentiate the two? Explain.
2. What method could you use to determine the structural stage of your embryo and support somatic embryogenesis, and not adventitious shoot regeneration?

Answer 1

1. Somatic embryos are an bipolar structure which can form shoot and root respectively.
2. There development occurs from somatic cells and hence are clones to their parents.
3. The developmesnt of the somatic embryos generally involves 3 broad stages which include,intial callus growth,middle callus phase, late embryonic callus phase.
4. The somatic embryogenesis is the formation of embryos vegetatively and results in the callus formation which can be differentiated from shoot.