Question

The purpose of the lactic acid in Lactophenol Cotton Blue is to:

1. preserve morphology

2. stain the fungal cells and spores

3. kill the mold

4. allow fermentation to take place

Answer 1

DISCUSSION

Lactophenol Cotton Blue:-This is a wet mount technique that is widely used for staining and observing the fungal structurals.

There are three components of the stain

1. Phenol:- It kills the fungus( organism)
2. Lactic acid:- It preserves the morphology of the fungal structure( hyphae, mycelium).
3. Cotton blue:- It is the stain used to stain the chitin layer of the fungal cell wall.

Option A:- CORRECT

Reason:- Lactic acid used in the staining preserves fungal structures of the fungus.

Option B:- Incorrect

Reason:- Cotton blue is the stain used for staining the cell and spore.

Option C:- Incorrect

Reason:- Phenol is a disinfectant that kills the fungal cells.

Option D:-Incorrect

Reason:-There is no role of fermentation reaction in wet-mount technique.