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When we add Trizol (or phenol:chloroform:isoamyl alcohol, same concept) to extract RNA from a sample, the...

When we add Trizol (or phenol:chloroform:isoamyl alcohol, same concept) to extract RNA from a sample, the RNA is in the aqueous phase.

True

False

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Answer #1

Answer :- TRUE

TRIzol solubilizes biological material and denatures protein. the addition of chloroform after solublization causes phase separation , where protein is taken out to the organic phase, DNA resolves at the interface, and RNA remains in the aqueous phase.

RNA stays in the aqueous phase since the pkA of RNA its groups is greater than that of DNA so RNA remains in the aqueous phase . This feature help separating one molecule without destroying the other.

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