Question

When we add Trizol (or phenol:chloroform:isoamyl alcohol, same
concept) to extract RNA from a sample, the RNA is in the
**aqueous** phase.

True

False

Answer #1

**Answer :- TRUE**

TRIzol solubilizes biological material and denatures protein. the addition of chloroform after solublization causes phase separation , where protein is taken out to the organic phase, DNA resolves at the interface, and RNA remains in the aqueous phase.

RNA stays in the aqueous phase since the pkA of RNA its groups is greater than that of DNA so RNA remains in the aqueous phase . This feature help separating one molecule without destroying the other.

**please give a thumbs up if the answer help you. Thank
you!**

Statistics sampling concept
1. we sample 7groups from the population and each group has
n=10,then we calculate the mean for each group . Finally, we use
the mean of each group to calculate the mean . The mean should be
closed to the population mean
2. we only sample one group but the n is large so the mean of
that group would be close to population mean as well
my question is when I should apply the first method...

1. How is complimentary DNA (cDNA) made from total RNA extract?
What type of enzyme and primer is needed? What are the advantages
of cloning a gene from its cDNA as opposed to the genomic DNA
sequence (introns)?
2. If a PCR reaction replicates both strands of the DNA each
cycle, this produces exponential amplification of the product. If
only one strand is replicated (as in a Sanger sequencing PCR
reaction) there is a linear growth in the product. Why...

true or false
When the level of confidence and sample proportion p remain the
same, a confidence interval for a population proportion p based on
a sample of n = 200 will be narrower than a confidence interval for
p based on a sample of n = 100.
When the level of confidence and the sample size remain the
same, a confidence interval for a population mean µ will
be narrower, when the sample standard deviation s is larger than...

Researchers A and B each have a random sample from the same
population, which has mean and a finite variance. Here are their
data:
Researcher A
Researcher B
Sample sizes
nA
nB
Sample means
Consider the following claims:
Claim 1. If nA > nB, the
estimator puts too much weight on researcher A’s
observations to be efficient.
Claim 2. If nA ≠≠ nB, the
estimator is biased.
Claim 3. If nA > nB, we can make the
estimator more efficient by...

1. If we add k indicator variables to a regression model for a
categorical variable with k levels, the regression tool will return
one coefficient estimate of 0.00 because:
a. The k variables are not independent b. The k variables are
independent
2. If we add up to 3rd order polynomial terms to a regression
model (e.g., x, x^2 and x^3) it will allow the relationship between
X and Y to change direction 3 times.
a. True b. False
3....

Consistency Concept says you must use the same (inventory
costing) method so financial statements can be compared from one
period to the next period. Group of answer choices
True or false

1. Insofar as we must generalize from a sample to a population,
the observed difference between the sample mean and the
hypothesized population mean a) can't be interpreted at face value.
b) might be due to variability or chance. c) might be real. d) is
described by all of the above
2. The advantage of a one-tailed test is that it increases the
likelihood of detecting a a) false null hypothesis. b) false null
hypothesis in the direction of concern....

When we convert scores from different measurements into
z-scores, we are allowed to compare the different measurements even
if they were measured on different scales. Group of answer
choices
True
False

1. When we add heat to ice at -10. deg C, what first happens,
and then if we continue to add more heat what else happens?
2. When the ice is heated to the melting point, and more heat is
added, what happens to the temperature?
3. Once the ice is all melted, as we add more heat, what happens
to the temperature? And this continues until what happens?
4. How many calories are required if 25.0 g of ice...

When performing inference on population means we require that
the data are normal and/or that the sample size is large (to use
the Central Limit Theorem) so that the sample means have a sampling
distribution that is at least approximately normal. The exact same
statement (in bold) holds for performing inference on population
variance(s) using the χ 2 or F-test.
(a) True
(b) False

ADVERTISEMENT

Get Answers For Free

Most questions answered within 1 hours.

ADVERTISEMENT

asked 6 minutes ago

asked 11 minutes ago

asked 14 minutes ago

asked 19 minutes ago

asked 19 minutes ago

asked 22 minutes ago

asked 30 minutes ago

asked 34 minutes ago

asked 34 minutes ago

asked 37 minutes ago

asked 37 minutes ago

asked 44 minutes ago