Question

In E. coli, the dam gene encodes a methylase that enzymatically adds a methyl group to...

In E. coli, the dam gene encodes a methylase that enzymatically adds a methyl group to adenines. In dam- mutants, this methylase activity is missing and adenines remain unmethylated. How do you think the absence of methylase activity affects the rate of spontaneous mutation in these mutants?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

DNA methylation is a posttranslational modification occur in E.coli due to dam gene which encode a special enzyme known as methylase

d = DNA

a=adenine

m=methylase

Through this process a methyl group bind to adenine, which help in a number of physiological process in the E. coli and also assist in the prevention of mutation and activate DNA repair pathway in the cell. When this gene is halted or absent completely then methylase enzymatic activity also nullify and as result there would not be DNA methylation through this process that make DNA vulnerable to the spontaneous mutation and DNA repair as well as replication pathway also compromised and there are fair chance to have mismatch during replication in such scenario.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
A theoretical operon (amo) in E. coli contains several structural genes encoding enzymes involved in the...
A theoretical operon (amo) in E. coli contains several structural genes encoding enzymes involved in the biosynthesis of a particular amino acid. Unlike the lac operon, in which the repressor gene is separate from the operon itself, for the amo operon, the repressor gene is found within the operon itself. When the end product amino acid is present, it binds to the repressor, and this complex binds to the operator, repressing the operon. In the absence of the amino acid,...
1) E. coli cells are growing in medium containing lactose but no glucose. Briefly describe the...
1) E. coli cells are growing in medium containing lactose but no glucose. Briefly describe the consequence of the following changes (one to two sentences for each condition): a. Addition of high concentration of glucose. b. A mutation that inactivates galactoside permease. c. A mutation that inactivates beta-galactosidase. d. A mutation that affects binding of CAP to c-AMP e. A mutation that affects binding of inducer to LacI f. A lac operator mutation that deletes all of the O1 2)...
In the absence of glucose E. coli can metabolize and grow on arabinose, a pentose sugar,...
In the absence of glucose E. coli can metabolize and grow on arabinose, a pentose sugar, using an inducible set of genes that are arranged in three groups on the chromosome (Fig shown). In one of these the araA, araB, andaraD genes encode enzymes for the metabolism of arabinose while the araC gene encodes a regulatory protein that binds adjacent to arabinose promoters and coordinates the expression of the genes in the arabinose operon. The other two groups of genes...
1. Currently you are studying threonine synthesis in E. coli. To find genes whose protein products...
1. Currently you are studying threonine synthesis in E. coli. To find genes whose protein products are important for threonine synthesis, you perform a mutagenesis to find mutants that require a source of threonine to grow. (a) Immediately after mutagenizing the bacteria, would you choose to grow the bacteria on rich media or on minimal media? Briefly explain your choice. Rich media. A mutant unable to make a particular amino acid will not be able to grown on media lacking...
1. Osmoregulation in E. coli Like most things we’ve talked about this semester, if eukaryotes do...
1. Osmoregulation in E. coli Like most things we’ve talked about this semester, if eukaryotes do it, bacteria do it too. Thus the existence of signal transduction pathways in bacteria should not surprise you. One of the best-studied signal transduction pathways controls how E. coli responds to changes in osmotic concentrations. EnvZ spans the cytoplasmic membrane and senses changes in osmolarity. OmpR is a transcription factor that works with EnvZ to adapt to these changing conditions. The signal passed between...
READ THE CASE STUDY AND ANSWER THE FOLLOWING QUESTIONS 2nd CASE: An Unexplained Death A 65-year-old...
READ THE CASE STUDY AND ANSWER THE FOLLOWING QUESTIONS 2nd CASE: An Unexplained Death A 65-year-old man of Scandinavian descent was rushed to the Emergency Room of your local hospital after a family member discovered him unconscious in his home. The woman who dialed “911” told the dispatcher that the man, her brother, was the local librarian of the past 10 years and had no spouse or children. She reported that they had spoken the day before, and he had...
Please answer the following Case analysis questions 1-How is New Balance performing compared to its primary...
Please answer the following Case analysis questions 1-How is New Balance performing compared to its primary rivals? How will the acquisition of Reebok by Adidas impact the structure of the athletic shoe industry? Is this likely to be favorable or unfavorable for New Balance? 2- What issues does New Balance management need to address? 3-What recommendations would you make to New Balance Management? What does New Balance need to do to continue to be successful? Should management continue to invest...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT