Question

A student properly flames her loop but then forgets to let it cool. Instead, she immediately inserts the hot loop into her broth culture. What is the most important issue associated with this mistake?

The hot loop may burn the student’s hand.

The hot loop may contaminate the broth culture.

The hot loop may distort the bacterial cells.

The hot loop may create aerosols when it touches the culture.

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Obtaining culture with a hot loop could, in fact, distort the bacterial cells. However, this is not the most important issue associated with the student’s mistake.

Answer 1

Ans. The inoculation loop is heated to sterilize it. It is set to cool before putting it into bacterial culture to inoculate. If we insert the red hot loop directly in the broth culture then its high temperature will evapoarte the culture medium containing some bactrial cell in it and it will form aerosols (a colloidal of small liquid droplets/ fine solid particles in air). These bacteria contaminated aerosols can be inhaled by the students or the aerosols can be diposited on student's body surface and can harm him/ her if they are pathogenic.

So last option is correct.