Question

Question 7 Use the illustration below to answer the next 5 questions.   A plasmid called pKSU1 is...

Question 7

Use the illustration below to answer the next 5 questions.   A plasmid called pKSU1 is shown on the left.  A piece of genomic DNA isolated from another organism is on the right.  The cut sites for three restriction enzymes are shown (SelI, HerII, HimIII) for both. Your goal is to insert the gene of interest from the genomic DNA into pKSU1.

Which restriction enzyme(s) would you digest the genomic DNA with to obtain a smaller fragment that contains the gene of interest (the entire gene)?

Question 7 options:

SelI

HerII

HimIII

all three together

Question 8 (1 point)

Which restriction enzyme(s) would you digest the pKSU1 with to insert the gene of interest from above into pKSU1?

Question 8 options:

SelI

HerII

HimIII

all three together

Question 9

Which restriction enzyme would you use to determine the orientation of the fragment containing the gene of interest in pKSU1?

Question 9 options:

SelI

HerII

HimIII

all three together

Question 10

How many fragments would be generated if pKSU1 was digested with SelI and HimIII?

Question 10 options:

1

2

3

4

Question 11

How many fragments would be generated if the genomic DNA was digested with SelI and HimIII?

Question 11 options:

3

4

5

6

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