Question

to get isolated colonies on a plate, only about 300 cells can be in the inoculum. what will happen if the cell density of the inoculum significantly excceds this number ?

Answer 1

when the cell density of the inoculum increases then it leads to increase in the number of bacterial cell in respect to less time.

when the number of cell increases then we cannot get separated colonies because high number of cells in inoculum leads to high number of formation of multiple colonies. so that the chances of formation of separated colonies does not occurs.

separation of colonies directly depends upon the number of cells in inoculum.

high number of cells in inoculum means high number of colonies which are totally integrated with each other.