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This is an essential cofactor for pyruvate dehydrogenase complex and whose deficiency results in this disease....

This is an essential cofactor for pyruvate dehydrogenase complex and whose deficiency results in this disease.

A.

Vitamin B1, Beriberi
B.

Vitamin B12, Anemia
C.

Vitamin C , Scurvy
D.

Biotin, No deficiency noted
E.

None of the above

What high energy intermediate in glycolysis is generated by Enolase?

a.

None of the above
b.

1,3-Bisphosphoglycerate
c.

Fructose-1,6-Bisphosphate
d.

2-Phosphoglycerate
e.

Phosphoenolpyruvate
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Ans:

A. Vitamin B1, beriberi

Explanation: The essential cofactor of pyruvate dehydrogenase complex are thyamine pyrophosphate, NAD+, FAD, CoA and lipoic acid.  Thyamine pyrophosphate is a derivatives of vitamin B1 and play important role as a cofactor in the oxidative decarboxylation of -keto acids. Beriberi is the major B1 deficiency syndrome. cobalamin is the derivatives of vitamin B12 and ascorbic acid is the derivatives of vitamin C. Biotin is a cofactor in carboxylation reaction.

e. Phosphoenol pyruvate.

Explanation: Phosphoenol pyruvate (PEP) is produced from 2-phosphoglycerate in the presence of the enzyme enolase.  2-phosphoglycerate is produced by the enzyme phosphoglyceromutase from 3-phosphoglycerate. 1,3 bisphosphoglycerate is formed by the enzyme glyceraldehyde-3-phosphate dehydrogenase and fructose-1,6- bisphosphate is formed by phosphofructokinase.

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