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The enzyme S-CoA is used twice during the Krebs Cycle. Why is this coenzyme useful, and...

The enzyme S-CoA is used twice during the Krebs Cycle. Why is this coenzyme useful, and what is the result of using it in each of those two occasions?

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Answer #1

In Kreb cycle Acetyl CoA act as a fuel.First step is condensation step.In this step 2 carbon acetyl group (of acetyl CoA) along with four carbon oxaloacetate form six carbon citrate molecule.In this step CoA is bound to sulfhydryl group ie. -SH group and leaves to combine with another acetyl group.Here, SH-CoA acts as citrate synthase.Play major role in Formation of Citrate.

Also in step 5 of Kreb cycle where cleavage of thioester bond of succinyl CoA is coupled with the phosphorylation of an ADP or GDP (substrate level phosphorylation).In this step CoA -SH act as CoA synthetase(Succinate Thiokinase).

So these two are functions of SH-CoA.

In first step SH-CoA helps in formation of Citrate and in 5th step it helps in formation of Succinate along with the release of energy in the form of ATP or GTP.

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