Question

Although the technical manual for the Bradford assay kit claims that very few lab reagents interfere...

Although the technical manual for the Bradford assay kit claims that very few lab reagents interfere with the assay, what control experiment can you do to determine whether a particular reagent interferes with the Bradford assay or not?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

If our protein is suspended in a solution of 0.5M NaOH (as for an example), and we are worried that the NaOH will interfere, then we should perform the Bradford Assay with no protein, but 0.5M NaOH instead. This will tell us if the NaOH interferes by producing a false positive.

If we think the NaOH can interfere the reaction by stopping it happening, then we should perform a Bradford Assay with a known amount of a protein (as for example albumin) in both water and in our 0.5M NaOH. If the results are different (like the 0.5M gives the smaller signal), then we will come to know that the NaOH is stopping the reaction happening properly (false negative).

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
A Dyson Fan is a very expensive, blade-less fan. There are a few different options available...
A Dyson Fan is a very expensive, blade-less fan. There are a few different options available (e.g., floor standing, heating fans, etc.).   The picture shows the desk-top version. These fans are advertised as fulfilling the following needs: reduce air buffeting, safety, easy to clean, and better speed control (a “dimmer” switch rather than discrete settings as in a regular fan). We want you to do two main things as part of your analysis of this product. First, assuming Dyson has...
Preparation of acetyl salicyclic acid (Aspirin) post-lab questions 1.) The FeCl3 test you performed on salicylic...
Preparation of acetyl salicyclic acid (Aspirin) post-lab questions 1.) The FeCl3 test you performed on salicylic acid, crude aspirin, and the purified aspirin gave different colors. Comment on the results you obtained in each of these tests in terms of colors and the functional groups present and what does that indicate about the purity of your purified aspirin? I got a dark purple color for saliclyc acid tube. "dark yellow" for crude aspirin tube, "llighter yellow" color for aspirin tube...
I have provided the information for the lab below. I want to see the chemical reactions...
I have provided the information for the lab below. I want to see the chemical reactions that take place during each step of the experiment. So in the procedures when a chemical was added i would like to see what the products and byproducts that were formed. I also want to see one over all reaction for this experiment to just get an overview of what exactly is happening during the experiment. Thank you:) Part B: Synthesis of [Co(NH3)5Cl]Cl2 Objective:...
As you saw from the lab PowerPoint slides last week, you will be doing a research...
As you saw from the lab PowerPoint slides last week, you will be doing a research study looking at ‘Aggression Priming” for your first paper. For this week’s discussion, I want you to discuss with your group what you think this study is about. What is the hypothesis? What theory does it come from? What do you predict will happen (do you expect something different than the hypothesis in the researcher instructions? If so, what and why?)? Do you think...
Acid-Base Behavior In addition to following the general safety rules, chemicals need to be handled properly....
Acid-Base Behavior In addition to following the general safety rules, chemicals need to be handled properly. In particular, two very important classes of compounds called acids and bases require special attention. These compounds are commonly used reagents in the laboratory; therefore, understanding their proper disposal is beneficial. Physical differences between acids and bases can be detected by the some of the five senses, including taste and touch. Acids have a sour or tart taste and can produce a stinging sensation...
READ THE CASE STUDY AND ANSWER THE FOLLOWING QUESTIONS 2nd CASE: An Unexplained Death A 65-year-old...
READ THE CASE STUDY AND ANSWER THE FOLLOWING QUESTIONS 2nd CASE: An Unexplained Death A 65-year-old man of Scandinavian descent was rushed to the Emergency Room of your local hospital after a family member discovered him unconscious in his home. The woman who dialed “911” told the dispatcher that the man, her brother, was the local librarian of the past 10 years and had no spouse or children. She reported that they had spoken the day before, and he had...
The Business Case for Agility “The battle is not always to the strongest, nor the race...
The Business Case for Agility “The battle is not always to the strongest, nor the race to the swiftest, but that’s the way to bet ’em!”  —C. Morgan Cofer In This Chapter This chapter discusses the business case for Agility, presenting six benefits for teams and the enterprise. It also describes a financial model that shows why incremental development works. Takeaways Agility is not just about the team. There are product-management, project-management, and technical issues beyond the team’s control. Lean-Agile provides...
Liebeck v. McDonalds Restaurants: The Original Coffee Product Liability Case James M. Dedman, IV Back in...
Liebeck v. McDonalds Restaurants: The Original Coffee Product Liability Case James M. Dedman, IV Back in 1994, Stella Liebeck v. McDonalds Restaurants became one of the most talked about lawsuits in American history. To this day, that New Mexico state court case is an essential component of any tort reform debate or discussion of litigation lore. At that time, and to this day, the thought of a fast food drive-thru customer spilling coffee on herself in her vehicle and later...
Muffler Magic* Muffler Magic is a fast-growing chain of 25 automobile service centers in Nevada. Originally...
Muffler Magic* Muffler Magic is a fast-growing chain of 25 automobile service centers in Nevada. Originally started 20 years ago as a muffler repair shop by Ronald Brown, the chain expanded rapidly to new locations, and as it did so Muffler Magic also expanded the services it provided, from muffler replacement to oil changes, brake jobs, and engine repair. Today, one can bring an automobile to a Muffler Magic shop for basically any type of service, from tires to mufflers...
Item 1 In the case below, the original source material is given along with a sample...
Item 1 In the case below, the original source material is given along with a sample of student work. Determine the type of plagiarism by clicking the appropriate radio button. Original Source Material Student Version But what are reasonable outcomes of the influence of global processes on education?While the question of how global processes influence all aspects of education (and who controls these forces) is multidimensional and not completely testable, there appear to be some theories of globalization as it...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT