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Although the technical manual for the Bradford assay kit claims that very few lab reagents interfere...

Although the technical manual for the Bradford assay kit claims that very few lab reagents interfere with the assay, what control experiment can you do to determine whether a particular reagent interferes with the Bradford assay or not?

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Answer #1

If our protein is suspended in a solution of 0.5M NaOH (as for an example), and we are worried that the NaOH will interfere, then we should perform the Bradford Assay with no protein, but 0.5M NaOH instead. This will tell us if the NaOH interferes by producing a false positive.

If we think the NaOH can interfere the reaction by stopping it happening, then we should perform a Bradford Assay with a known amount of a protein (as for example albumin) in both water and in our 0.5M NaOH. If the results are different (like the 0.5M gives the smaller signal), then we will come to know that the NaOH is stopping the reaction happening properly (false negative).

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