Question

Complete the table to summarize the following modes of RNA-mediated regulation.

Mechanism	Prokaryotes, eukaryotes, or both?	Molecular target? (DNA, RNA, polypeptide)	Process affected? (Transcription, Translation)	Direction of effect? (Increase, Decrease, Both)	Cis- or trans- acting?
Antisense RNA (asRNA)					Depends on the source of the asRNA
RNAi by miRNA
RNAi by siRNA		DNA
RNAi by siRNA		RNA
Riboswitches

Answer 1

Mechanism	Prokaryotes, eukaryotes or both?	Molecular target? (DNA, RNA, polypeptide)	Process affected? (Transcription, translation)	Direction of effect? (Increase, decrease, or both)	Cis- or trans- acting?
Antisense RNA	both	RNA (specifically mRNA)	translation	both	depend on the source of the asRNA
RNAi by miRNA	eukaryotes	RNA (multiple mRNA targets)	post transcription	decrease	trans- acting
RNAi by siRNA	eukaryotes	RNA (only one mRNA target)	post trancription gene silencing	decrease	trans- acting
Riboswitches	prokaryotes	RNA	premature termination of transcription(attenuation) ot translational inhibition	both	cis- acting