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Delbeccos Modified Eagle Media the base media used to culture HeLa cells provides what necessary for...

Delbeccos Modified Eagle Media the base media used to culture HeLa cells provides what necessary for proper cell growth?

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Answer #1

DMEM is a basal medium and contains no protein and no growth promoting agents it has almost twice the concentration of amino acids and four times the amount of vitamins.it required suplimentation to be a complete medium.

Original formulation contained 1000m/l of glucose, further variation with 4500mg /l of glucose

It suplimemted with 5_10 % of fetal bovine serum, DMEM utilities a sodium bicarbonate buffer system, because it maintain the required PH,power Media is required by addition of 3.7g/l of sodium bicarbonate upon dissolving in water.

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