Question

What is a gene knockout (KO)? Indicate whether the gene expression is regulated at transcriptional and/or translational level. Briefly explain your answer.

Answer 1

1. Gene Knockout- It is the genetic technique in which the one organisms that is gene is made up the incoperative means knockout the organism. It usually known to study the gene function and also it investigate the effect of the gend loss.

2. The eukaryotic gene expression is regulated in the transcriptional level and the RNA processing takes place in the nucleus and the time of the translation of Protein it takes place in cytoplasm.

3. The prokaryotic gene expression occurs both transcriptional and translation simultaneously in the cytoplasm and in transcriptional level regulation takes place.