Question

please answer the following regarding PCR

1. Detail why there must be 2 distinct primers in the reaction. (2)

2. For the nucleotides that are added to the PCR reaction, sketch out a sample of one and provide its complete, specific name Do not electronically copy and paste an image from another source. (3)

3. Given the large human genome of 3 million base pairs, why would one need to make copies of DNA?

Answer 1

Q1. the two primer are used in PCR becauses, 2 primers are desigined to match the segments of DNA you want to copy . through the complementry base pairing , one primer attached to the top strands at one end of your segment of interest and other primer attachees to the bottom of strand at the other end.

Q2. answer is in image

Q3.the DNA is copyed becauses , to have haploid reference of genome. in human genome every individule has unique gene .