Question

You identify a protein that may play a role in microtubule-based transport. Propose a series of experiments that would aid you in confirming your assessment that this protein has a role in microtubule-based transport.

Please explain the purpose of each step.

Answer 1

Exp1-

Logic: Use Microtubule depolymerizing agent like Nocodazole. It will tell microtubule are essential for given protein. mediated transport

EXP: Treat the cells with either nocodazole or DMSO and check by microscopy that GIven protein can move or not.

Result: If the protein can not move, it suggests that Microtubules are essential for movement.

EXP2

Logic: Use SiRNA to deplete the given protein and use labelled cargo. It will tell cargo will not move when proein is depleted

Exp: Use control or Protein specific siRNA to deplete the protein and check the Cargo movement.

result: I expect cargo proteins will not move in protein depleted condition compared to control.

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