Question

1. A researcher isolates three new antibiotics that act by inhibiting GTP hydrolysis on bacterial translation factors IF-2, EF-Tu, and EF-G, respectively. Predict at what stage/step would translation be blocked by each antibiotic. Provide a brief explanation for each of your predictions.

Answer 1

1) When IF2 is blocked by the inhibitor, then the initiator tRNA can not bind to the correct position in ribosomes. Because the IF2 factor directs the initiator tRNA to the P site in ribosomes. Hence the initiation step of translation is inhibited

2) When the EF- Tu is blocked, then there is not found entry of amino acyl tRNA to the A site of ribosome. Because EF-Tu binds to the GTP and make a complex with tRNA. And directs to the A site. Hence the elongation step is inhibited.

3) When EF G is inhibiter, then there is not found movement of ribosomes to the three nucelotides along mRNA molecule. Because EF binds with GTP and allows the ribosomes shifting to the three nucelotides in mRNA. Henec the elongation step is inhibited.

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