Question

you transform your Arabidopsis plants with Agrobacterium tumefaciens, using kanamycin antibiotic resistance as a selection marker. you harvest the seeds from you transformed plants and now you want to find out which seeds were transformed by agrobacterium. explain how younwill now select to find plants that have been successfully transformed. Answer should be concise but specific what materials you would use, what controls you would have and how would you be able to tell which plant is tranformed

Answer 1

To select for transformed seeds against non transformed seeds, we will use three nutrient media plates -

1. Positive control = only contains nutrient media

All transformed and non transformed seeds will grow.

2. Negative control = nutrient media + kanamycin

Only transformed seeds will grow because they contain gene for resistance to kanamycin.

So by using positive control and negative control, we can select for the seeds which are transformed.

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