Question

How do PCR and qPCR differ?

Answer 1

Quantitative PCR (qPCR) is used to detect, characterize and quantify nucleic acids.
qPCR (typically green), fluorescent labeling allows the quantification of the amplified DNA molecules
Application - quantification of nucleic acid standards, quantification of next-gen sequencing Libraries and quantification of gene expression.

PCR
PCR, DNA is amplified by 3 repeating steps: denaturation, annealing and elongation.
No fluorescent is use.
No Quantitative analysis
Application - Sequencing, Genotyping, Cloning