Question

A microbiologist is trying to isolate a pathogen expected to be present in very small numbers from a stool specimen in which there are abundant normal microbiota. Which strategy would most likely accomplish this purpose?

Culture on enriched media

Enrichment culture followed by culture on selective agar

Culture on differential media

Culture in an anaerobe jar

Answer 1

answer is 2 enrichment culture followed by culture on selective agar

This method is designed to inhibit unwanted or contaminating bacteria and help to recover pathogen from a mixture of bacteria. Though selective media are agar based, enrichment media are liquid based in consistency. Both of these media serve the same purpose. Any agar media can be made selective media by adding certain inhibitory agents that don’t distress the pathogen of interest.

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