Which of the following glycolytic enzymes is regulated by both allosteric regulation and reversible phosphorylation?
Group of answer choices
phosphoglyceromutase
pyruvate kinase
glyceraldehyde-3-phosphate dehydrogenase
phosphoglycerate kinase
cyclic AMP-dependent protein kinase
Answer: Pyruvate kinase
Pyruvate kinase is used in the last step of glycolysis and its regulation is important while making pyruvate. They are regulated by both by allosteric regulation and reversible although its function is affected or regulated by various minor factors one important effector factor is FBP or fructose 1,6 bisphosphate which works allosterically to regulate the enzyme.
While there are various isozymes for PK one of the form is M2 which exists in both dimer and tetramer form. The tetramer form acts as the active enzyme but it can be regulated reverse phosphorylating it into dimer and making it inactive.
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