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Question 1 What is the process of separating DNA from the other proteins? What techniques are...

Question 1

What is the process of separating DNA from the other proteins? What techniques are used to accomplish this?

  

Question 2

How do we isolate the DNA so we have just DNA and can now use other techniques to identify it?

  
  
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Answer #1

1) treatment of protease enzyme which degrade protein will cause removal of protein while DNA remain intact.

2) we can isolate DNA by using phenol:chloroform: isoamyl alcohal solution. Protein get precipitate at interphase of organic solvent and water while DNA dissolve in aquous phase of water. By seperating aquous water phase, DNA is precipatting by adding isopropanol or 100% ethanol that leads to seperation of DNA.

We can use agarose gel electrophoresis for identifying or taking spectrophotometer reading at 260nm which shows specific absorbance- reading by DNA only.

Thanks..!

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