Question

After purifying DNA, an absorbance value at 260nm was found to be 1.50. Based on the calculation method described in your lab manual, what is the concentration in ng/uL?

true or false

Answer 1

for double-stranded DNA.

The absorbance of 1 at 260 nm corresponds to the concentration of  50µg/ml.

Absorbance is directly proportional to concentration.

A/C= a constant

1/50=constant

so 1.5/x=1/50 ( x is the concentration of DNA sample with absorbance 1.5)

x=1.550

=75 microgram/mL

1 microgram= 10^3 nanogram

and 1 mL= 1000 uL

so 75 microgram/mL= 7510^3 nanogram/1000 microliter=75ng/microliter

so the answer is 75ng/microliter