Question

A graduate student implants a tissue engineered scaffold into a rat. After two weeks, the cells in the construct are not viable enough even though there is no scar tissue or immune reaction to the construct. Name three parameters that could be influencing transport in and out of the cell.

Answer 1

* Biocompatibility :- scaffold adheres & integrates to the surroundings of native tissue of rat.

* 3D structure of scaffold :- this may have maintained cell exact phenotype of rat tissue.

* Surface chemistry and topography of scaffold tissue:- this may have promoted cell anchorage and differentiation; ECM production.

* mechanical properties :- this allow mechanical integrity that associated with the reconstruction of tissue.

* Degradation of scaffold tissue.