Question

Briefly describe the method you would you use to separate the following types of membrane-bound proteins...

Briefly describe the method you would you use to separate the following types of membrane-bound proteins from the lipid membrane?

a. Integral Membrane Proteins - _______________

b. Peripheral Membrane Proteins (that are not lipid-linked) - _______________

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Integral membrane proteins are those which are completely embedded inside the membrane and span both the surfaces of the membrane. They can be extracted out from the membrane only by disrupting the complete structure of membrane. It is possible by using crude treatment like treating the plasma membrane with detergents. Detergent break down the bonds present between phospholipids in the plasma membrane thereby extracting out integral membrane protein.

Peripheral membrane proteins are those which are present towards the one surface of the membrane. Therefore they can be easily isolated from the membrane without disturbing the structure. This is possible by using mild treatment like high concentration of salt or pH.

Please give a good rating.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
1a. Cholesterol is not used for which of the following: Making the membrane less fluid Making...
1a. Cholesterol is not used for which of the following: Making the membrane less fluid Making the membrane more fluid Bile acids and salts synthesis Steroid hormone synthesis 1b. In which part of a membrane would cholesterol be found? Covalently attached to the polar head groups of the phospholipids. Bound to peripheral protein molecules. Embedded in the fatty acid layer in the middle of the lipid bilayer. Attached to the cytoplasmic domain of integral membrane proteins.
Which of the following types of molecules are hydrophilic and therefore excluded from the hydrophobic portion...
Which of the following types of molecules are hydrophilic and therefore excluded from the hydrophobic portion of the phospholipid bilayer? Select one: a. cholesterol b. peripheral membrane proteins c. integral membrane proteins d. transmembrane proteins e. phospholipids
Can you separate a mixture of the following three proteins using membrane electrophoresis? Protein A has...
Can you separate a mixture of the following three proteins using membrane electrophoresis? Protein A has a pI > pH of the run buffer, Protein B has a pI < the pH of the run buffer, and Protein C has a pI = pH of the run buffer. If so, describe the movement of each protein.
There are 9 different types of membrane-bound adenylate cyclase (AdCy) enzyme. If you had a receptor...
There are 9 different types of membrane-bound adenylate cyclase (AdCy) enzyme. If you had a receptor that was linked to AdCy type II via a Gi type of G protein (i.e. a G protein for which the ? subunit is inhibitory), what would happen to signalling through this receptor if adaptation in the brain to an addictive drug caused the cell to express AdCy type VII rather than AdCy type II? Explain your answer.
Briefly describe when you would use the extraction (shake-flask) method vs. the LC-MS method to measure...
Briefly describe when you would use the extraction (shake-flask) method vs. the LC-MS method to measure LogP. Include considerations about sample size and polarity.
Which of the following would happen if a mutation knocked out the receptor for lysosome bound...
Which of the following would happen if a mutation knocked out the receptor for lysosome bound proteins in the golgi? A. The proteins normally found in the lysosome would go to the mitochondria instead B. The proteins normally found in the lysosome would accumulate in the golgi C. The proteins normally found in the lysosome would accumulate in the Rough ER D. The proteins normally found in the lysosome would go to the nuclear membrane instead
Lorazepam is a positive allosteric modulator with high affinity for GABAA receptors, membrane bound, ligand-gated channel...
Lorazepam is a positive allosteric modulator with high affinity for GABAA receptors, membrane bound, ligand-gated channel permeable to chloride. Answer the following questions regarding the administration of lorazepam for the patient in this case study. a. Identify two membrane-bound proteins present that significantly influence the resting membrane potential of an excitable cell. For each, identify the ions transported and the type of membrane transport (active/passive). b. Describe how membrane-bound ligand-gated channels transiently alter membrane permeability to a particular ion, and...
) Briefly describe the three types of bilingualism 2) How you would include in your environment...
) Briefly describe the three types of bilingualism 2) How you would include in your environment and materials 3) What you would do if a child's language was not spoken in the classroom 4) Describe embedded assessment and what it would look like in your classroom or program. 5) Anything that really resonates with you, stands out to you or surprises you about working with Dual Language Learners.
You want to isolate a functional integral membrane protein from the cell to study its channel...
You want to isolate a functional integral membrane protein from the cell to study its channel activity. What method would you use to isolate the integral membrane potential? By adding SDS By using Beta-mercaptoethanol By boiling By using a mild-detergent By adding high concentration of salt
1) Briefly describe the three types of bilingualism 2) How you would include in your environment...
1) Briefly describe the three types of bilingualism 2) How you would include in your environment and materials 3) What you would do if a child's language was not spoken in the classroom 4) Describe embedded assessment and what it would look like in your classroom or program. 5) Anything that really resonates with you, stands out to you or surprises you about working with Dual Language Learners. PreviousNext
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT