Question

A)

Complete the following diagram by giving the template sequence, the mRNA sequence and peptide sequence.(The genetic code is provided below)Complete the following diagram by giving the template sequence, the mRNA sequence and peptide sequence.(The genetic code is provided below)

Coding:            5’ GCT GAT CAG CTG ACC GAA GAA CAG ATT 3’

Template:     3’ ___ ___ ___   ___ ___ ___ ___   ___ ___ 5’

mRNA:            5’ ___ ___ ___ ___ ___ ___ ___   ___ ___ 3’

Protein:                  N    _      _         _       _       _       _       _         _      _     C

B) Do you think this sequence is of the beginning of the gene, the middle or the end and why?

Answer 1

Coding Strand:          5’ GCT GAT CAG CTG ACC GAA GAA CAG ATT 3’

Template Strand:     3’ CGA CTA GTC GAC TGG CTT CTT GTC TAA 5’

mRNA:            5’ GCU GAU CAG CUG ACC GAA GAA CAG AUU 3’

Peptide sequence:          5' Ala - Asp – Gln – Leu – Thr – Glu – Glu – Gln – Ile 5’

This sequence is the middle of the gene.

AUG codes for start codon and UAA, UAG and UGA codes for stop or end codon. Since none of these is present to illustrate the beginning or end of the code, the given peptide sequence is the middle of the gene.