Question

Growth Curves and Logistic Equation

You are working with a culture of Chinese Hamster Ovary Cells (CHO). Given:

-Specific growth rate=1.2 days^-1

-Initial cell population=10^5 cells

-Maximum population that can be sustained by the environment=10^8 cells

-There is a lag phase, 0 days<t<1 days, during which no growth occurs. Exponential growth begins at t=1 day.

Write an equation that describes the number of cells as a function of time. Plot cell concentration vs time on a semilog plot. On your graph, label the following regions: lag phase, exponential growth, stationary phase.

Answer #1

Lag phase from day 0 to day 4

Exponential phase from day 5 to 10

Stationary phase from day 11 to day 14.

And this graph was plotted using above information such as specific growth rate and initial count final count etc.

**dX/dT=uX where u is specific growth rate and X is number
of cells at t time**

another equation between bacterial numbers and time

G = t

3.3 log b/B

where

t = time interval in hours or minutes

B = number of bacteria at the beginning of a time interval

b = number of bacteria at the end of the time interval

G=generation time

Therapeutic proteins are expressed in mammalian Chinese
Hamster Ovary Cells (CHO), which are grown in rich media broth that
often is supplemented with proteins. In this cell culture broth, a
receptor containing an Fc group (known as an Fc-fusion protein) was
expressed at a titer of 1g receptor/L of cell culture broth. This
particular receptor is used to treat Rheumatoid Arthritis by acting
as a decoy receptor for Tumor Necrosis Factor alpha and decreasing
its binding to its native receptor...

A pure culture in exponential growth has a bacterial
concentration of 64x10
7 cells/ml. If the bacteria have a
doubling time of 1 hour, how long ago was the cell
concentration 8x107 cells/ml?

1) In a bacterial culture, the number of bacteria, f(t), is
defined by the equation f(t) = Be0.02t where B is a constant, and t
is the time elapsed in minutes the initial number of bacteria is
1000 . Note: initial number of bacteria means the number at the
start or t = 0 A. compute for the constant B B. determine the
number of bacteria after 50 minutes
2) In a certain bacterial culture the number of bacterial cells...

Can you paraphrase the following rationale for me:
Male Reproductive System
1. Which of the following accurately describes
spermiogenesis?
a. Occurs before puberty
b. Involves stem cells, meiosis, and spermatogenesis
c. Involves cytodifferentiation of early spermatids
d. Occurs in diploid cells
e. Results in the formation of primary spermatocytes
Rationale:
Spermatogenesis, the first part of sperm production involves
mainly mitosis and meiosis and is followed by spermiogenesis, the
final differentiation process occurring in the haploid male germ
cells. Spermatogenesis begins...

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