Question

In a preparation of isolated mitochondria, the succinate is used as the sole substrate in the...

In a preparation of isolated mitochondria, the succinate is used as the sole substrate in the presence of ADP and Pi to stimulate the transfer of electrons and the synthesis of ATP. An inhibitor X of the respiratory chain is added. Then, we observed an accumulation of the cofactors of the respiratory chain either in oxidized form or in reduced form as indicated in the table below. Based on these results, indicate from the list below, which inhibitor is used.

Cofactors Forms
FAD/FADH2 Reduced
Ubiquinone/Ubiquinol Reduced
Cytochrome C Reduced
O2/H2O Oxidized
Inhibitors Roles
Rotenone Inhibits the complex I
Malonate Inhibits the complex II
Antimycine Inhibits the complex III
Cyanide Inhibits the complex IV
Oligomycine Inhibits the complex V

Homework Answers

Answer #1

Option D, cyanide, inhibitor of complex IV

All the ETC components before complex IV will remain in reducing state while O2 and H20 ( which is reduced by complex IV) will be in oxidized state.

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