Question

You visualize your protein of interest in small punctate dots within the cell. You believe the...

You visualize your protein of interest in small punctate dots within the cell. You believe the protein could be traveling in a vesicle between the Golgi and ER. What type(s) of vesicles could your protein be in?

To confirm the type of vesicle your protein is traveling in you GFP tag a protein that will help you identify the vesicle type. Please state what protein you will tag and, if found together with your protein of interest, what vesicle your protein is traveling in. (

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Answer #1

Protein travels from endoplasmic reticulam to Golgi or Golgi to ER through coat protein 2 formed vesicles or coat protein 1 formed vesicles respectively. Hence the protein of interest may be present together with either of the protein

If we tag coat protein 2 with GFP and if it binds with proteins and travel , we can identify through the luminescence and if not found we can also tag the other coat protein 1 with red flourescent protein to identify in which vesicle it travels properly

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