Question

1)Which of the following will be affected in an opposite manner (inhibited by one and stimulated by the other) by cAMP-dependent protein kinase and AMP-activated protein kinase? Select all that apply

Phosphofructokinase-2 in ischemic heart

Lipolysis in adipose tissue

Glycogenesis in skeletal muscle

Glycogenolysis in liver

Cholesterol biosynthesis in liver

Gluconeogenesis in liver

Glucose uptake via GLUT 4 transporters

1)Which of the following will be activated by phosphorylation of key enzymes by protein kinase A (cAMP dependent protein kinase? (select all that apply)

Gluconeogenesis in liver

Glucose uptake via GLUT 4 transporters

Glycogenolysis in liver

Glycogenesis in skeletal muscle

Cholesterol biosynthesis in liver

Phosphofructokinase-2 in liver

Lipolysis in adipose tissue

3)Which of the following are treatments are currently widely used for a number of inborn errors of metabolism in humans? (select all that apply)

Ex vivo gene replacement

Infusion with stem and or progenitor cells

In vivo gene replacement using CRISPR-Cas9 modified cells

Oxygen transplantation

Use of RNA interference

Cofactor administration

Dietary manipulation

3)Match the pathway with its most highly regulated enzyme.

Fructose 1,6 biphosphate phosphatase

Glucose-6-phosphate dehydrogenase

Glycogen synthase

Phosphofructokinase 1

Acetyl CoA carboxylase

Carbamoyl phosphate synthetase

A.Glycogenesis

B. Glucogenesis

C.Urea cycle

D.Fatty acid synthesis

E.Glycolysis

F.Pentose phosphate pathway

4)Which of the following are DNA repair processes which are required to make the required gene edits after use of one of the gene editing systems? (select all that apply)

Alkytransferase catalyzed direct repair

Homologus recombination

Base excision repair

Nonhomologous end joining repair

Nucleotide excision repair

Answer 1

2). Activated by phosphorylation of key enzymes of protein kinase A - Glycogenolysis in liver

Glucose uptake via GLUT 4 transporters

Phosphofructokinase kinase 2 in liver

Gluconeogenesis in liver

3). Ex vivo gene replacement

Dietary manipulation

In vivo gene replacement using CRISPR-CAS 9 modified cells

Oxygen transplantation

Cofactor administration

Infusion with stem or progenitor cells

  

4). Glycogenesis - Glycogen synthase

Glucogenesis - Fructose 1,6 biphosphate phosphatase

Urea cycle - Carbamoyl phosphate synthetase

Fatty acid synthesis - Acetyl co A carboxylase

Glycolysis - Phosphofructokinase - 1

Pentose phosphate pathway - Glucose 6 phosphate dehydrogenase

5). Homologous recombination

Base excision repair

Nucleotide excision repair