Question

Even though the system that expresses protein is different between humans and bacteria, what is the principle and how does the plasmid DNA express GFP in human cells?

This is the question of Biochemical lab assignment of expressing plasmid DNA on human cells using "transfection".

Answer 1

Answer: The plasmid DNA is an extrachromosomal molecule which can exist independently. They can be inherited from one generation to other or from one system to other and therefore used to transfer the genetic information.The method of transferrinf these plasmid DNA to mammalian system is called transfection which can be performed by many methods such as lipofection, microinjection etc.

Even though the system that express proteins is diifferent between humans and bacteria, the plasmid express GFP in human cells because the plasmid carrying the GFP fuction as individual geneome. They can express their protein independently and there is no need of insertion into the host genome. The plasmid containing the GFP will be present in the mammalian cell after the transfection but it will not incorporate in the host genome. It will be present as independent extrachromosomal genome.