Question

after centrifugation , what is your sample ? serum or plasma?

Answer 1

Usually plasma is used for the samples after centrifugation process. Because plasma is obtained  from whole blood that is not left to clot, and contains serum along with clotting factors unlike the serum. To obtain plasma for the process, a whole blood sample is collected in test tubes and treated with anticoagulants. Following up with the centrifugation, cells are removed and plasma supernatant remains. Discussing about the process briefly, centrifugation is done for 15 minutes at 2,000 x g depletes platelets in the plasma sample. The resulting supernatant is so called plasma. Following up with the centrifugation, it is important to transfer immediately the liquid component i.e., plasma into a clean polypropylene tube using a Pasteur pipette.