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Why do we use binding buffer or CP buffer in different protocol to purify DNA from...

Why do we use binding buffer or CP buffer in different protocol to purify DNA from E.Coli?

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Answer #1

Citrate-phosphate buffer or CP buffer. It consists of citric acid and dibasic sodium phosphate.

The lysate sample containing DNA in the centrifuge tube is buffered at specific pH and getting separated from other cell components. Buffers keep the pH stable. When cells are lysed open they release many types of compounds that can change pH which could alter the properties of the target molecule. Including a buffer prevents this and keeps the pH to something similar to that in the cell. Please bear in mind that DNA is denatured by increasing the pH and it can be damaged in acidic conditions. On centrifugation the DNA is getting adsorbed to the bind which can be eluted our using eluting buffer.

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