Question

1. During replication, what would be the consequences of the loss of functions of;
   • i. single-stranded binding proteins,
   • ii. DNA ligases,
   • iii. DNA topoisomerases, and
   • iv. DNA helicases?
   • During replication, what would be the consequences of the loss of functions of;
     • i. single-stranded binding proteins,
     • ii. DNA ligases,
     • iii. DNA topoisomerases, and
     • iv. DNA helicases?

Answer 1

SSBP-These bind to the newly separated individual DNA strands ,keeping the strands separated by holding them in place.In their absence the separated strands might form the helix again

DNA ligase-Okazaki fragments are joined by these enzymes. In their absense a continous daughter strand would not be formed

DNA topoisomerase-In absense of topoisomerase the DNA becomes hypernegatively supercoiled. The supercoiling tension is reduced by these enzymes

DNA helicase-These unwind the DNA by utilizing the energy derived fron triphosphates. They break hydrogen bonds between the two complementary strands.In their lack of function DNA unwinding wont be possible