SSBP-These bind to the newly separated individual DNA strands ,keeping the strands separated by holding them in place.In their absence the separated strands might form the helix again
DNA ligase-Okazaki fragments are joined by these enzymes. In their absense a continous daughter strand would not be formed
DNA topoisomerase-In absense of topoisomerase the DNA becomes hypernegatively supercoiled. The supercoiling tension is reduced by these enzymes
DNA helicase-These unwind the DNA by utilizing the energy derived fron triphosphates. They break hydrogen bonds between the two complementary strands.In their lack of function DNA unwinding wont be possible
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