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If I pirefied DNA by kit for some E.Coli samples , should I send the actual...

If I pirefied DNA by kit for some E.Coli samples , should I send the actual samples with the purified DNA samples to sequence or not ?

Some samples have less than 50 nucleic acid. What should I do with them to get high concentration of nucleic acid ?
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Answer #1

Yes u can send the samole as such but only on a condition that they should be processed imidiatly .if the sample will not processed imidiatly then the sample must be refrigerated at a temprature less than 10 degree celcius. U must know that e coli samples must not be held more than 6 hours between collection and initiation of analysis.samples must be processed within 2 hours of reaching the lab

If some.samples has less number of nuclic acid then u can subject those samples to PCR that is polymerase chain reaction which will give u exactly same sample nucleic acid with increase in number .

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