Question

What limits the shotgun sequencing approach for larger genomes like human?

	a.	lack of appropriate DNA primers
	b.	presence of thousands of repetitive sequences, confounding assembly
	c.	the human genome is too complex for computer programs
	d.	shotgun sequencing does not work with chromatin
	e.	nothing; it was used to sequence the human genome

Third-generation sequencing differs from second-generation sequence because

	a.	It uses fluorophores to identify bases in a sequence
	b.	it sequences a single molecule of DNA
	c.	it processes many sequences simultaneously
	d.	A camera images the fluorescence

Answer 1

The limitations of the shotgun sequencing approach for larger genomes like humans include: the need for sophisticated computer programs, a reference genome or a genome map to use as a reference for the vast amount of DNA that is being sequenced and presence of repetitive sequences will be difficult to assemble. Hence the correct option is:

b. presence of thousands of repetitive sequences, confounding assembly

Third generation sequencing differs from second generation sequencing because it sequences a single molecule of DNA whereas the second generation sequencing does sequencing of a stretch of DNA molecules. Hence the correct option is:

b. it sequences a single molecule of DNA

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