Question

Provide the correct mRNA sequence for the following DNA sequence, and then translate this into the proper amino acid sequence for the protein the gene would encode:

5′ ATG GAA CGC CTT TAC ATC GTG TGG CAT TAT AGC CAA CGC GTT TAA 3′ Coding

3′ TAC CTT GCG GAA ATG TAG CAC ACC GTA ATA TCG GTT GCG CAA ATT 5′

Answer 1

The first strand is given as the coding strand, so the other strand is the template srand for transcription. The mRNA sequence produced from the template strand will be

5' - AUG GAA CGC CUU UAC AUC GUG UGG CAU UAU AGC CAA CGC GUU UAA- 3'

The aminoacid sequence of the protein encoded by the gene will be

N- Methione - Glutamic acid - Arginine - Leucine - Tyrosine - Isoleucine - Valine - Tryptophan - Histidine - Tyrosine - Serine - Glutamine - Arginine - Valine - C