Question

There are many steps and tasks necessary to conduct a DNA Analysis project to determine the phylogenetic relationship of organisms. Below are 10 key steps and tasks necessary, though not in the correct order. Put them in the correct order (assuming ‘everything works’ on the first attempt). Some steps/tasks are used more than once.

• DNA Sequencing
• PCR Purification
• Submit Research Permit approval
• DNA quantification
• Phylogenetic Analysis
• Collect tissue
• Gel Electrophoresis
• DNA Extraction
• PCR
• Sequence analysis

Answer 1

For a DNA Analysis project to determine the phylogenetic relationship of organisms, follow the below given order

1. Submit Research Permit approval
2. Collect tissue
3. DNA Extraction
4. Gel Electrophoresis
5. DNA quantification
6. PCR
7. PCR Purification
8. Gel Electrophoresis
9. DNA quantification
10. DNA Sequencing
11. Sequence analysis
12. Phylogenetic Analysis

First you have to submit a research proposal and permit approval, then collect samples and extract DNa from the tissue.After DNa extraction run a Gel Electrophoresis to detect the presence of DNA and obtain a rough estimate of the quantity of DNA from the gel. Then run the PCR and after PCR amplification, purify the PCR for downstram process. You can run a Gel Electrophoresis of the amplified PCR product and can quantify the PCR product if necessary. Next step is DNA Sequencing followed by Sequence analysis and Phylogenetic Analysis