Determine the N:C ratio naturally found in P. fluorescens by mass (50% carbon and 15% nitrogen). Carbon and nitrogen comprise 50% and 15% of the biomass by weight. Determine the N : C ratio in terms of number of atoms. #N atoms : #C atom?
Atomic mass of carbon- 12
Atomic mass of nitrogen- 14
Let the biomass be W in weight.
Weight of carbon= 50% of W= 0.5W
Weight of nitrogen = 15% of W= 0.15W
Now no of atoms of carbon= weight of carbon/ atomic mass of carbon × 6.023× 10^23
No of atoms nitrogen= weight of nitrogen/ atomic mass of nitrogen × Na( Avogadro number)
No of atoms of N= 0.15W/14= 0.01071W × Na
No of atoms of C= 0.5W/ 12 × Na= 0.0416W × Na
Ratio of N:C atoms= 0.0107/0.0416
Ratio of N:C atoms= 107/416
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