1. Which of the following is CORRECT concerning transcription of E. coli genes?
A. Transcription termination may involve rho factor
B. Sigma factor is required for elongation of a RNA chain
C. Melting or unwinding of a DNA template is achieved by a helicase
D. Both DNA strands in a gene are transcribed from one promoter
2. Math the items: 1. mRNA, 2. rRNA, 3. tRNA, 4. exon, 5. intron
A. Synthesized by RNA Polymerase lll in eukaryotes:
B. Is NOT modified in prokaryotes:
C. Forms a loop structure(lariat) during splicing:
D. Has non-DNA encoded " poly A" added to the 3' end:
3. Which of the following transcription processes is possible only in prokaryotes?
A. Regulation of the amount of transcription from a gene
B. Regulation of where transcription begins
C. Co-transcriptional translation
D. Transcription generating a new RNA that grows in the 5' --->3' direction
Answer:-1) rho factor is required for the termination of the transcription process.
Sigma factor isrequired for the initiation of the transcription process . Unwinding and melting of the the DNA is done by the DNA dependent RNA polymerase. Only one strand of DNA is transcribed in the process of transcription.
Answer:-2) tRNA synthesized by RNA polymerase 3 in in eukaryotes.
Introns forms a loop structure during splicing.
m RNA is not modified in prokaryotes.
Answer :- 3) Co transcription translation is possible only in prokaryotes.As the Transcription occurs in the DNA and DNA is present in the nucleus whereas translation occurs in the cytoplasm . There is no division between cytoplasm and nucleus in the prokaryotes therefore transcription and translation occurs simultaneously in the prokaryotes such as bacteria .
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