Question

The Bradford assay is especially sensitive to arginine residues.  If you have a protein with a high...

The Bradford assay is especially sensitive to arginine residues.  If you have a protein with a high percentage of arginine

  1. How will this affect your absorbance values?

.

B. What might you do to get more accurate protein concentration values?

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Answer #1

A. If the percentage of arginine is high in protein then absorbance values will be higher because coomassie dye binds to basic amino acid residues, arginine, lysine and histidine and forms the protein-dye complex (blue coloured complex) which is measured directly by measuring the absorption at 595 nm to quantify the protein concentration.

B. We can dilute the protein sample and then perform the Bradford assay to get more accurate protein concentration values if the protein concentration of sample is higher than the concentrations in the linear range of standard curve.

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