Question

1. make a table to compare and contrast gram and endospore stains . ex: 1 % percent stain, counter stains and mordant.
2. discuss acidic and basic stains

Answer 1

1) Following are the similarities and difference between the two types of staining procedures:

S.no	Gram stain	Endospore stain
1	Primary stain is malachite green	Primary stain is crystal violet
2	Iodine acts as mordant	Heating acts as mordant
3	Ethyl alcohol acts as decolorizier	Decolorizer not needed
4	0.5% saffranin is used as counterstain	0.5% saffranin is used as counterstain

5. Endospore staining method helps distinguish endospores from vegetative cells whereas gram stain helps distinguish gram positive form gram negative based on their, peptidoglycan content.