Question

What are the steps for map-based cloning in crop plants?

Answer 1

step 1.

Identify a marker tightly linked to your genein a ''large''mapping population

step 2

find a YAS or BAC clone to which the marker probe hybridizes

step 3

create new markers from the large-insert clone and determine if they co-segregate with your gene

step 4

if necessary,re-screen the large-insert genomic library forother clones and search for co-segregating markers

step 5

identify a candidate gene from large-insert clone whose markers co-segregate with the gene

step 6

perform genetic complemention to rescue the wild type phenotype

step 7

sequence the gene and determine if the function is known